AHMED CHAHDI

[email protected]

410-953-9994

EDUCATION:

- Ph.D., Cellular Biology and Cellular and Molecular Pharmacology, Louis Pasteur University, Strasbourg, France, 1997.

- B. Biochemistry, Limoges University, France, 1993.

PROFESSIONAL EXPERIENCE:

03/2022-Present                              Research Scientist, Nemours Children’s Hospital

10/2018-11-2021                              Assistant Scientist, Department of Medicine, University of Miami.

02/2016- 12/2017                             Research Assistant Professor, Internal Medicine, University of Utah.

08/2014- 08/2015                             Research Assistant Professor, Hormel Institute, University of Minnesota.

02/2010- 10/2013                             Research Assistant Professor of Medicine, University of Maryland, Baltimore, Department of Medicine, Division of Gastroenterology.

06/2001- 11/2009                             Research Associate, Research Scientist I, Medical College of Wisconsin, Department of Medicine, Division of Nephrology.

01/1998- 05/2001                             Postdoctoral Fellow, National Institutes of Health, National Heart, Lung and Blood Institute, laboratory of Molecular Immunology, section on intracellular signaling.

PUBLICATIONS

1. Ahmed Chahdi, Carolina Jorgez , Jill A Rosenfeld, Neetu Neetu, Abhishek Seth. Androgen receptor ubiquitination links KCTD13 to genitourinary tract defects. FASEB J. 39(4) 2025.
2. Carolina J Jorgez, Ahmed Chahdi, Hunter Flores, Marisol O'Neill, Abhishek Seth. Role of Kctd13 in modulating AR and SOX9 expression in different penile cell populations. Andrology. 13(5), 2025.
3. Ahmed Chahdi, Carolina Jorgez, Abhishek Seth. Regulation of androgen receptor stability by the β₁ Pix/STUB1 complex. FASEB J. 38(2), 2024.
4. Kunrong Cheng, Ahmed Chahdi, Shannon M Larabee and Jean-Pierre Raufman. Muscarinic receptor agonist-induced βPix binding to β-catenin promotes colon neoplasia. Sci Rep. 13(1), 2023.
5. Abhishek Seth, Armando Rivera, A. Chahdi, In-Seon Choi, Olga Medina-Martinez, Shaye Lewis, Marisol O'Neill , Alex Ridgeway, Joshua Moore, Carolina Jorgez, Dolores J Lamb. Gene dosage changes in KCTD13 result in penile and testicular anomalies via diminished androgen receptor function. FASEB J. 36 (11), 2022.
6. K. Cheng, SM. Larabee, M. Tolaymat, A. Chahdi and JP. Raufman. Targeted intestinal deletion of Rho guanine nucleotide exchange factor 7, βPix, impairs enterocyte proliferation, villus maturation, and mucosal defenses in mice. American J Physiol Gastrointest Liver Physiol. 320 (4): G627-G643, 2021.
7. Said AH, Hu S, Abutaleb A, Watkins T, Cheng K, Chahdi A, Kuppusamy P, Saxena N, Xie G, Raufman JP. Interacting post-muscarinic receptor signaling pathways potentiate matrix metalloproteinase-1 expression and invasion of human colon cancer cells. Biochem J. 474(5):647-665, 2017.
8. A. Chahdi and JP. Raufman. The Cdc42/Rac Nucleotide Exchange Factor β1Pix Modulates b-catenin Transcriptional Activity in Colon Cancer Cells: Evidence for Direct interaction of b₁Pix with β-catenin. J. Biol. Chem. 288(47):34019-29, 2013.
9. JP. Raufman, K. Cheng, N. Saxena, A. Chahdi, A. Belo, S. Khurana and G. Xie. Muscarinic receptor agonists stimulate matrix metalloproteinase 1-dependent invasion of human colon cancer cells. Biochem. Biophys. Res. Commun. 415(2):319-24, 2011.
10. A. Belo, K. Cheng, A. Chahdi, J. Shant, G. Xie, S. Khurana and J-P Raufman.

Muscarinic receptor agonists stimulate human colon cancer cell migration and invasion. American Journal of Physiology- Gastrointestinal and Liver Physiology. 300(5): G749-60, 2011.

11. T.S. Pavlov, A. Chahdi, D.V. Vachugova, A. Vandewalle, O. Pochynyuk, A. Sorokin, and A. Staruschenko.

Endothelin-1 inhibits the epithelial Na+ channel through β1Pix/14-3-3/Nedd4-2. J. Am. Soc. Nephrol. 5:833-843, 2010.

12. A. Chahdi and A. Sorokin. The role of β1Pix/caveolin-1 interaction in endothelin signaling through G a subunits.  Biochem. Biophys. Res. Commun. 391:1330-1335, 2010.
13.  A. Chahdi and A. Sorokin. Endothelin-1 induces p66Shc activation through EGF receptor transactivation: Role of β1Pix/Ga_i3 interaction. Cell. Signal. 22:325-329, 2010.
14. A. Chahdi and A. Sorokin. Endothelin-1 couples βPix to p66Shc: Role of βPix in cell proliferation through FOXO3a phosphorylation and p27^kip1 down regulation independently of Akt. Mol. Biol. Cell. 19:2609-2619, 2008.
15. A. Chahdi and A. Sorokin. PKA-dependent phosphorylation modulates β1Pix-GEF activity through 14-3-3b binding. Mol. Cell. Biol. 28:1679-1687, 2008.
16. A. Chahdi and A. Sorokin. Endothelin 1 stimulates β1Pix-dependent activation of Cdc42 through the Gas pathway. Exp. Biol. Med. 231: 761-765, 2006.
17. A. Chahdi, B. Miller and A. Sorokin. Endothelin 1 induces β1Pix translocation and Cdc42 activation via protein kinase A-dependent pathway. J. Biol. Chem. 280: 578-584, 2005.
18. W.S. Choi, T. Hiragun, Y.M. Kim, H.P. Kim, A. Chahdi, E. Her, J.W. Han and M. A. Beaven. Activation of mast cells is dependent on tyrosine phosphorylation of phospholipase D2 by Fyn and Fgr. Mol. Cell. Biol. 24: 6980-6992, 2004.
19. A. Chahdi, A. Sorokin, M.J. Dunn and Y. Landry. The Rac/Cdc42 guanine nucleotide exchange factor b₁Pix enhances mastoparan-activated Gi-dependent pathway in mast cells. Biochem. Biophys. Res. Commun. 317: 384-9, 2004.
20. A. Chahdi, W.S. Choi, Y.M. Kim and M.A. Beaven. Mastoparan selectively activates phospholipase D2 in cell membranes. J. Biol. Chem. 278:12039-45, 2003.
21. A. Chahdi, W.S. Choi, Y.M. Kim and M.A. Beaven. Serine/threonine protein kinases synergistically regulate phospholipase D1 and 2 and secretion in RBL-2H3 mast cells. Mol. Immunol. 38: 1269-1276, 2002.
22. W.S. Choi, A. Chahdi, Y.M. Kim, P.F. Fraundorfer and M.A. Beaven. Regulation of phospholipase D and secretion in mast cells by protein kinase A and other protein kinases. Ann. N. Y. Acad. Sci. 968:198-212, 2002.
23. A. Chahdi, P.F. Fraundorfer and M.A. Beaven. Compound 48/80 activates mast cell phospholipase D via hetrotrimeric GTP-binding proteins. J. Pharmacol. Exp. Ther. 292: 122-130, 2000.
24. L. Daeffler, A. Chahdi, J.P. Gies and Y. Landry. Inhibition of GTPase activity of G_i proteins and decreased agonist affinity of M₂ muscarinic acethylcholine receptors by spermine and methoctramine. Br. J. Pharmacol. 127: 1021-1029, 1999.
25. A. Chahdi, L. Daeffler, J.L. Bueb, J.P. Gies and Y. Landry. The M₂ muscarinic receptor antagonist methoctramine activates mast cells via pertussis toxin-sensitive G proteins. Naunyn-Schmiedeberg’s Arch. Pharmacol. 357: 357-362, 1998.
26. A. Chahdi, M. Mousli and Y. Landry. Substance P-related inhibitors of mast cell exocytosis act on G-proteins or on the cell surface. Eur. J. Pharmacol. 341 : 329-335, 1998.
27. A. Chahdi, L. Daeffler, J.P.Gies and Y. Landry. Drugs interacting with G protein a subunit: selectivity and perspectives. Fundam. Clin. Pharmacol. 12: 121-132, 1998.